Nurse Lana


The DAO muscle is responsible for pulling the corners of the mouth downward, which can create a sad or downturned appearance. By injecting in the area 1cm lateral and 1cm down from the corner of the mouth, the aim is to relax the activity of the DAO muscle and allow the corners of the mouth to lift, resulting in a more uplifted and rejuvenated appearance.

In the field of cosmetic procedures, the DAO muscle can be targeted with treatments such as Botox injections to temporarily relax its activity. By inhibiting the action of the DAO muscle, Botox can help elevate the corners of the mouth and create a more uplifted and rejuvenated look. This can be especially beneficial for individuals who have a naturally downturned mouth or who wish to correct the appearance of marionette lines, which are the vertical lines that extend downward from the corners of the mouth.


Technique 1

Placement: 1cm lateral and 1cm down from the corner of the mouth

Units: 3 to 5 per sides

Depth: Full Needle (Deep)

Here are some tips for performing the technique:

  1. Ask your client to make a sad face or frown to activate the targeted muscle.
  2. Locate the muscle by gently pinching the area to identify its position.
  3. Instruct your client to relax their facial muscles completely.
  4. Align the needle and inject straight into the desired location, targeting the muscle of concern.

Combining Neurotoxins with Dermal Fillers

The combination of treating the Depressor Anguli Oris (DAO) muscle with dermal fillers can be beneficial in certain cases. It is typically considered when there is a noticeable volume loss or asymmetry in the lower face, particularly around the corners of the mouth.

When the DAO muscle is overactive, it can contribute to a downturned or sad appearance. Botox or other muscle relaxants can be used to temporarily weaken the muscle and lift the corners of the mouth. However, in some instances, the volume loss in the area may also need to be addressed to achieve a balanced and harmonious result.

Dermal fillers can be used to add volume and restore symmetry to the lower face. By strategically placing the fillers in the surrounding areas, such as the marionette lines or the corners of the mouth, the downward pull of the DAO muscle can be counteracted. This combination approach can help to achieve a more youthful and lifted appearance.

It’s important to note that the decision to combine DAO treatment with dermal fillers should be made on an individual basis.


The following disclaimer is provided for informational purposes only and should not be considered as professional advice or a substitute for medical consultation. The online course on Advanced Techniques of Neurotoxins and Dermal Fillers, taught by Nurse Lana and affiliated with Sparkle Lifestyle & MediSpa, is designed to provide educational content and training in the field of aesthetic procedures involving neurotoxins and dermal fillers.

It is important to note that participating in this course does not qualify individuals as licensed healthcare professionals or certify them to administer neurotoxin or dermal filler treatments. The course is intended to enhance knowledge and understanding of advanced techniques related to neurotoxins and dermal fillers.

Participants should be aware that the proper administration and application of neurotoxins and dermal fillers require appropriate medical training, qualifications, and expertise. It is strongly recommended that individuals consult with a qualified healthcare professional and obtain appropriate licensure and certifications in their respective jurisdictions before attempting to perform these procedures. The course content is not intended to endorse or encourage the unauthorized practice of medicine or aesthetic procedures without proper qualifications.

The information provided in the course is based on current knowledge and practices in the field but may not reflect the most recent updates or advancements. Nurse Lana, as the course instructor, has made reasonable efforts to ensure the accuracy and reliability of the content; however, she cannot guarantee its completeness, accuracy, or suitability for any specific individual or situation.

Participants are responsible for their own actions and decisions related to the use of neurotoxins and dermal fillers and should exercise caution, skill, and professional judgment when applying the techniques learned in the course. Nurse Lana, Sparkle Lifestyle & MediSpa, and the platform hosting the course shall not be held liable for any damages, injuries, or adverse outcomes arising from the misuse, misinterpretation, or improper application of the techniques discussed in the course.

It is strongly advised that participants seek hands-on training, mentorship, and supervision from qualified healthcare professionals experienced in administering neurotoxins and dermal fillers to gain the necessary skills and competence before attempting to perform such procedures independently.

By enrolling in the online course on Advanced Techniques of Neurotoxins and Dermal Fillers, participants acknowledge that they have read, understood, and agreed to the terms of this disclaimer and accept full responsibility for their actions and decisions in the field of neurotoxins and dermal fillers.

Please consult with a qualified healthcare professional or medical authority for personalized advice, diagnosis, or treatment related to neurotoxins, dermal fillers, or any other medical condition or aesthetic concern.